 ELISA (Enzyme-Linked ImmunoSorbent Assay)
ELISA/EIA is quantitative detection of the presence of an antibody or an antigen in a sample. Either antigens or antibodies are affixed to a surface. Then after the binding, an enzymatic substrate is added to produce a visible signal, which indicates the quantity of antigen in the sample.
To find the apropriate products, please refer to the product lists.Product Lists。
Elispot
Eli-spot is a technique used for identification and enumeration of cytokine-producing cells at the single cell level. By Eli-spot technique, frequency analysis of rare cell populations is now relatively easy. It is commonly used in many European ans American laboratories for diagnostic and research purpose now.
Either a monoclonal or polyclonal capture antibody is coated aseptically onto a microplate. These antibodies are chosen for their specificity for the analyte in question. After incubation, cytokine (or other cell product of interest) secreted by activated cells is captured locally by the coated antibody on the high surface area. After washing, a biotinylated polyclonal antibody specific for the chosen analyte is added to the wells. This antibody is reactive with a distinct epitope of the target cytokine.The colored end product (a spot, usually a blackish blue) typically represents an individual cytokine-producing cell. The spots can be counted manually (e.g., with a dissecting microscope) or using an automated reader to capture the microwell images and to analyze spot number and size.
Except Enzymatic Elispot, there are also Dual Enzymatic Elispot and Dual Fluorospot products. To find the approprate products quickly, please refer Product Lists。
Rapid Screen Tests
Based on Sandwhich ELISA, these are quantitative detection of multiple drugs or drug metabolites in blood. The tests only take about five minutes. There are three methods: Panels 、Cards and Dipsticks. To find appropriate products, plesae refer to Product List。
Multiplex Assays
■ Using xMAP techniques can simultaneously detect 33 analytes
■ Products including: Adipokine, Apolipoprotein, Cytokine/Chemokine, Hormone analytes for Rat/Human/Mouse/
■ Require only 5-25μl sample
■ Suitable for serum, plasma and tissue samples
■ High percision
■ Reducing researching time and budget
To find appropriate products, plesae refer to Product List。
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